Molecular Genetics of Bacterium

Molecular Genetics of Bacteria fulfills the need for a comprehensive, primary textbook in bacterial molecular genetics. It is ideally suited as a textbook for advanced undergraduate level courses and as background reading for graduate level courses. It is also recommended reading for scientists in all fields of biology, many of which depend upon the concepts and techniques covered in this book. While the book concentrates of necessity on the bacterium Escherichia coli, about which most is known, it also uses examples from other bacteria as appropriate. The material in each chapter has been substantially updated to reflect exciting developments in the field of bacterial molecular genetics and its relationship to other fields, including eukaryotic cell biology and development. Recent years have been a period of consolidation" in biology with many seemingly diverse areas being discovered to be different manifestations of the same phenomenon, and these relationships have been emphasized. It has also been a period of major technical advances, including genomics and microarrays, which are also covered in the book. The second edition retains the same organization and style as the first edition, and the order of presentation of the topics has been largely unchanged as this order was popular with most instructors using the textbook. As before, each chapter contains a chapter summary, a set of discussion questions to encourage speculation, problems (with answers), and suggested readings, all of which are updated to reflect advances in the field. It also retains the use of "boxes" to present related material of interest to each topic without breaking the continuity of the text. The secondedition will continue to serve as an important text for all courses in bacterial molecular genetics and as background for courses in molecular biology and biotechnology.

This manual encompasses an integrated series of molecular biology laboratory exercises that involve the cloning and analysis of the bioluminescence "(lux)" genes from the marine bacterium "Vibrio fischeri." The manual is divided into discrete units with each demonstrating one or more aspects of the cloning project. The manual is based on one of nature's most fascinating biological phenomenon: the biological production of light. This results in a recurrent theme of interest and makes the project very relevant to interdisciplinary topics such as fish symbiosis, biochemistry, biophysics, etc. Includes instruction in the basic techniques of modern molecular biology: DNA isolation and analysis, DNA restriction, agarose gel electrophoresis, ligations, transformation of recombinant DNA, preparation and screening a genomic library, restriction mapping, Southern blotting, hybridization, DNA sequencing, pulsed field gel electrophoresis. Designed for a one semester course in Molecular Biology. Also appropriate for a molecular biology component of Microbial Genetics, Genetics, Biochemistry, or Advanced Microbiology courses.

Molecular genetics - Molecular genetics is the field of biology which studies the structure and function of genes at a molecular level. Molecular genetics employs the methods of genetics and molecular biology.

Ecological genetics - Ecological genetics is the study of genetics on an ecological scale. While molecular genetics studies the structure and function of genes at a molecular level, ecological genetics (and the related field of population genetics) studies wild populations of organisms.

Classical genetics - Classical genetics consists of the techniques and methodologies of genetics that predate the advent of molecular biology. A key discovery of classical genetics in eukaryotes, was genetic linkage.

Conservation genetics - Conservation genetics is a science that aims to apply genetic methods to deal with the maintenance, loss, and restoration of biodiversity. Researchers involved in conservation genetics come from a variety of fields including population genetics, molecular ecology and biology, evolutionary biology, and systematics.

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